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galK [2019-04-04 14:36:58]
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galK [2019-04-04 14:36:58]

galactokinase
locus
BSU38200
pI
5.15
mw
43.36 kDa
protein length
390 aa Sequence Blast
gene length
1173 bp Sequence Blast
function
galactose utilization
product
galactokinase
essential
no
ec
2.7.1.6
synonyms
ipa-35d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.8|Utilization of galactose]

    • Gene

    Coordinates
    3,920,638 3,921,810

    The protein

    Catalyzed reaction/ biological activity

  • ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate (according to Swiss-Prot)

    • Protein family

  • GalK subfamily (according to Swiss-Prot)

    • Structure

  • [PDB|1PIE] (from ''Lactococcus lactis'', 43% identity, 60% similarity) [Pubmed|12796487]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK]-[gene|591428EA1C3D40322BB942429607AF61A90BAF41|galT]
    description
    [Pubmed|9353933]

    regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]
    • view in new tab

    genes
    [gene|C3D87DAA8DB124D50D2C2B2D424C9608C0DE067F|ywcC]-[gene|FD30C4E458E63E8D6A932B351D4224F1AACD4B1B|gtcA]-[gene|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK]-[gene|591428EA1C3D40322BB942429607AF61A90BAF41|galT]
    description
    [Pubmed|22383849,22893383]

    regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]
    • view in new tab

    Biological materials

    Mutant

  • BKE38200 ([gene|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGCAATCACCTTTCTG, downstream forward: _UP4_AGAGAACTAAAGGGGGAGTG
  • BKK38200 ([gene|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGCAATCACCTTTCTG, downstream forward: _UP4_AGAGAACTAAAGGGGGAGTG

    • References

  • 9353933,10666464,22383849,22893383